도청신도시 예천 1,102 : Lactobacillus...

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  Lactobacillus acidophilus ATCC 4356의 16S-23S rRNA intergenic spacer region sequence 특성과 균체 섭취에 의한 Salmonella enteritigis 감염시 쥐의 체중변화에 미치는 영향(Lactobacillus acidophilus ATCC 4356의 16S-23S rRNA intergenic spacer region sequence 特性과 菌體 攝取에 依한 Salmonella enteritigis 感染時 쥐의 體重變化에 미치는 影響 =Charateristics of 16S-23S rRna intergenic spacer region sequence of Lactobacillus acidophilus ATCC 4356 and Feeding Effects on the Change in Body weight of Rat in Salmonella enteritidis infection) [學術저널] : 지보면 신풍리 출신인 윤영호(尹永皓, 1941- , 中央大 敎授) 등(배진성)의 논문으로, 중앙대학교 식량자원연구소(中央大學校 食糧資源硏究所 =Food and Resources Institute, Chung-Ang University)에서 2002년에 발행한 <식량자원연구소 논문집(食糧資源硏究所 論文集 =JOURNAL OF FOOD AND RESOURCES INSTITUTE)> Vol.14 No.1 40-49쪽(10쪽 分量)에 수록되어 있다. 주제어 weight of spleen liver body weight contamination level of Salmonella sequence of 16S-23S rRNA intergenic spacer region이다. [초록] : Lactobacillus acidophilus의 16S-23S rRNA intergenic spacer region의 특성과 Lactobacillus spp. 급여에 의하여 Salmonella enteritidis 감염 이후 체중저하에 대한 보호효과를 주요 장기 중에 오염된 생균수 및 장기 중량 변화성향을 측정한 결과는 다음과 같다. Lactobacillus acidophilus의 16S-23S rRNA intergenic spacer region의 sequence를 측정하고 gene bank에 등록된 균주의 sequence와 비교한 결과 homology의 차이를 나타내었다.

  Lactobacillus spp. 급여 이후 Lactobacillus enteritidis를 challenge 이후 체중 변화는 4일 경과 후부터 체중 감소현상을 보였으며 체중 감소율이 가장 낮은 균주는 Lb. helveticus CU 631이었으며 균주간의 차이를 나타내었고, 간과 비장에 Salmonella enteritidis 오염 생균수는 Lactobacillus spp. 급여군이 대조군보다 낮은 성행을 보였고 간장 및 비장의 중량은 대조군보다 낮은 성향을 보였다. =16S-23S rRNA intergenic spacer region sequence and effects of Lactobacillus acidophilus ATCC 4356 against typical enteritis causing pathogen Salmonella enteritidis were determinded. Changes in the weight of body, spleen, liver and the contaminati on level of Salmonella enteritidis in the vital organ after challenge have been determined. The sequence of 16S-23S rRNA intergenic spacer region of Lactobacillus acidophilus could be utilized as a strain identification, those sequences comparison showed difference in homology. A rapid decline in body weight after seven days of challange with Salmonella enteritidis were observed in control mice, the protective effects from the decline in body weight by feeding Lactobacillus spp was most prominent in Lb. helveticus CU 631 and in Lb./ acidophilus, more numerous viable number of Salmonella enteritidis in the tissues of spleen and liver and heavier average organ weight in control mice(unfed with Lactobacillus spp.) than lactobacillus spp. fed mice was observed. [목차] : Ⅰ. 서론/ Ⅱ. 재료 및 방법/ 1. 사용균주 및 보관/ 2. Lactobacillus acidophilus의 16S-23S rRNA intergenic spacer region의 specific sequence 측정/ 3. Lactobacillus spp.급여에 의한 쥐에 Salmonella enteritidis 감염시 체중 및 비장 간장 중량 과 병원균오염도 측정/ 4. 통계분석/ Ⅲ. 결과 및 고찰/ 1. Lactobacillus spp.의 16S-23S rRNA intergenic spacer region의 sequence 비교/ 2. Lactobacillus spp.의 Salmonella enteritidis 감염에 대한 보호효과(keris 2006)

  Lactobacillus spp.와 Bacillus coagulans의 glutathione sulphydryl 함유율과 항산화 활성(Lactobacillus spp.와 Bacillus coagulans의 glutathione sulphydryl 含油率과 抗酸化 活性 =Glutathione sylphydrate contents and Antioxidant activities of Lactobacillus spp. and Bacillus coagulans) [學術저널] : 지보면 신풍리 출신인 윤영호(尹永皓, 1941- , 中央大 敎授) 등(변정열)의 논문으로, 중앙대학교 유전공학연구소(Institute of Genetic Engineering Chung-Ang University)에서 2003년에 발행한 <유전공학연구논집(遺傳工學硏究論集 =JOURNAL OF GENETIC ENGINEERING RESEARCH)> Vol.16 No.1 31-38쪽(8쪽 分量)에 수록되어 있다. 주제어 GSH level antioxidant effect이다. [초록] : 활성산소의 세포막 파괴효과로부터 보호작용과 항산화 활성이 강한 균주를 선별하기위하여 33균주에 대한 세포내 glutathione 함유율과 linolcic acid 산화억제활성을 측정하였다. Lactobacillus casei HY2782 25.15 u㏖/g으로 가장 높은 함유율을 보이고 있으며 Lactobacillus rhamnosus CU01, Lactobacillus plantarum CU03 이 14.0u㏖/g 으로 통계적인 유의성을 나타내는 수준으로 높은 함유율을 나타내었다.

  포자형성균주중의 glutathione 함유율측정결과 Lactobacillus sporogenes CU815, sporolactobacillus inulinus ATCC13538 은 Bacillus coagulans 균주의 GSH 함유율 보다 유의성이 인정되는 높은 함유율을 나타내었다./ L. acidophilus ATCC4356, L. casei HY2782, L. brevis CU06 L. helveticus CU631은 Lactobacillus spp 중 높은 항산화 활성을 나타내었고 Bacillus coagulans의 항산화 활성범위는 6.02~51.72%로 나타났으며 Bacillus coagulans균주의 항산화 활성이 Lactobacillus spp.의 산화억제활성보다 높은 성향을 나타내었다. =The antioxidative ability on the basis of reduced glutathione sulphydryl level, the inhibition activities of linoleic acid peroxidation of cell free extract of Lactobacillus spp and Bacillus spp. have been determined; Lactobacillus casei HY 2782 contained the highest level of GSH among the probiotic strains with 25.1μmole/g. Significanly high level of GSH occured in the intracellular cell free extract of Lactobacillus rhamnosus CU01, Lactobacillus plantarum CU03 The antioxidant activity and inhibition of linoleic acid peroxidation of cell free extract of Lactobacillus spp and Bacillus spp by thiobarbituric acid(TBA) assay have been shown to be significantly differed depending on the strains(P>0.01). Intracellular cell free extracts of L. acidophilus ATCC4356, L. casei HY2782, and strains of Bacillus coacillus revealed a significantly intensive inhibitory activity in the linoleic acid peroxidation reactions. Spearmans' rand correlation between inhibitory activity on linoleic acid peroxidation and cellular GSH levels of Lactobacillus spp was analysed and the correlation quotient was 0.65 which means a significant positive correlation.(keris 2006)

  Lactobacillus spp.와 Bifidobacterium spp.의 세포벽 분해효소 Autolysin의 특성(Lactobacillus spp.와 Bifidobacterium spp.의 細胞壁 分解酵素 Autolysin의 特性 =Characteristics of Cell Wall Hydrolyzing Enzyme of Lactobacillus spp. and Bifidobacterium spp.) [學術저널] : 지보면 신풍리 출신인 윤영호(尹永皓, 1941- , 中央大 敎授) 등(이재후)의 논문으로, 한국축산학회에서 1998년에 발행한 <한국축산학회지(Korean Journal of animal science)> Vol.40 No.1 43-50쪽(8쪽 分量)에 수록되어 있다. 소장된 대학 도서관은 중앙대(안성중앙), 우석대(중앙), 대구대(중앙)이다. [초록] : Studies were carried out to investigate the nature of cell wall hydrolyzing enzymes of Lactobocillus spp. and Bifidobacterium spp. which were utilized as starter organisms for fermented dairy products, the following results were obtained;/ Thirteen out of twenty-one strains of Lactobacillus and Bifidobacterium strains showed intensive clear cell wall hydrolyzing zone around the colonies on MRS agar plate containing 0.2% lyophilized Micorcoccus luteus cells./ Cell wall hydrolyzing enzymes were isolated by SDS-PAGE and the cell wall hydrolyzing bands were detected on M. luteus cell containing gels. Each strains varied in molecular weight of cell wall hydrolyzing enzyme and in the number of cell wall hydrolyzing enzyme. L. casei YIT 9018 possess a single cell hydrolyzing enzyme of 76kDa./ Optimum temperature and pH of the cell wall hydrolyzing enzyme of L. casei YIT 9018 were 37℃ and pH 6.0. Monovalent cations inhibited the enzymatic activity at above 0.1M concentrations and divalent cations inhibited the enzymatic activity extensively.(keris 2006)

  Lactobacillus spp.와 Bifidobacterium spp.의 Pulsed Field Gel Electrophoresis에 의한 Genomic DNA의 특성(Lactobacillus spp.와 Bifidobacterium spp.의 Pulsed Field Gel Electrophoresis에 依한 Genomic DNA의 特性 =Characteristics of the Genomic DNA of Lactobacillus spp. and Bifidobacterium spp. by Pulsed Field Gel Electrophoresis) [學術저널] : 지보면 신풍리 출신인 윤영호(尹永皓, 1941- , 中央大 敎授) 등(이재후 백영진)의 논문으로, 한국축산학회에서 1998년에 발행한 <한국축산학회지(Korean Journal of animal science)> Vol.40 No.1 51-58쪽(8쪽 分量)에 수록되어 있다. 소장된 대학 도서관은 중앙대(안성중앙), 우석대(중앙), 대구대(중앙)이다. [초록] : Characteristics of genomic DNA of Lactobacillus spp. and Bifidobacterium spp. were determined by pulsed field gel electrophoresis and the following results were obtained;/ Four enzymes, Sma I, Apa I Not I and Sfi I were identified as suitable for generation of relatively few numbers of distinct fragments from the Lactobacillus spp. genomes, whereas five enzymes, Sma I, Apa I, Sfi I and Dra I were suitable for Bifidobacterium spp. genomes./ The genome sizes of Lactobacillus spp. ranged from 1.S8Mb to 3.31Mb, the genome sizes of L. brevis ATCC 8287, L. fermentum ATCC 14931, L. casei YIT 9018, L. plantarum KCTC 1048 and L. delbruekii subsp. delbruekii ATCC 9469 were estimated to be 1.93Mb, 1.98Mb, 2.84Mb, 2.81M6 and 1.82Mb, respectively./ Those of Bifidobcxterium spp. ranged from 1.85Mb to 2.83Mb. The genome sizes of B. bifrdum ATCC 29521, B. longum ATCC 15707, B. infantis ATCC 15697 and B. bifidum CU 1370 were estimated to be approximately 1.85Mb, 2.83Mb, 1.86Mb and 1.94Mb, respectively.(keris 2006)